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| Monoclonal Antibody to IL-1beta (Clone IMG43N4C4) |
| Catalog No |
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IMG-6823A |
| Contents |
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0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Mouse IgG1, Kappa |
| Clone |
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IMG43N4C4 |
| Purification |
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Protein G Chromatography is used to purify this antibody |
| Species React |
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Human |
| Host |
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Mouse |
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| Application
Western blot analysis: use antibody at 1-5 ug/ml
Storage
Store IL-1beta antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.
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Background
IL-1beta (Interleukin-1 beta, IL-1b) is a potent inflammatory cytokine which has been well studied. Produced as a precursor, especially in myeloid cells and monocytes/macrophages, IL-1beta is processed by cleavage with caspase-1, or ICE, from the 31 kD inactive precursor to the 18kD active mature form secreted from cells as an end-result of the inflammasome complex and activation sequence (1, 2). In response to various TLR ligands, myeloid cells or macrophages activate NLRP3 inflammasomes and secrete IL-1beta. The rate of IL-1beta processing has been correlated to redox state or levels of ROS generated (3, 4). IL-1beta is directly involved in a variety of diseases -–auto-inflammatory diseases, acute inflammatory attacks such as ischemic injury in myocardial infarcts or in chronic inflammatory processes of atherosclerosis or Type 2 diabetes (5, 6). The ability to detect and quantitate active IL-1beta is critical for understanding how to regulate its activity as a therapeutic target. |
Antigen
Full-length recombinant human IL-1beta protein was used as immunogen for this antibody.
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Application Notes
View additional IL-1 beta antibodies and products here |
Genebank Info (Protein): NP_000567
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Gene ID: 3553
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SwissProt: P01584
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Western blot analysis of IL-1beta in full-length recombinant protein using IL-1beta antibody at 3 ug/ml.
IMGENEX’s goat anti-mouse Ig HRP secondary antibody (20101) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Ghayur, T et al 1997 Caspases-1 Processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production Nature 386: 619-623
2. Gu, Y et al 1997 Activation of interferon –gamma inducing factor mediated by interleukin-1 converting enzyme Science 275: 206-209
3. Carta, S et al 2011 The rate of production of interleukin-1b Secretion in different myeloid cells varies with the extent of Redox Response to Toll-like Receptor triggering. J. Biol. Chem. 286: 27069-27080
4. Tassi, S et al 2009 Pathogen-induced interleukin-1beta processing and secretion is regulated by a biphasic redox response. J. Immunol. 183: 1456-1462.
5. Dinarello, CA 2011 A clinical perspective of IL-1b as the gatekeeper of inflammation. Eur. J. Immunol. 41: 1203-1207
6. Hanamsagar, R, V Torres and T Kielian 2011 Inflmmasome activation and IL-1b/IL-18 processing are influenced by distinct pathways in microglia. J. Neurochem. 119: 736-748
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