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| Polyclonal Antibody to SOCS3 |
| Catalog No |
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IMG-6838A |
| Contents |
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0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype |
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Rabbit IgG |
| Purification |
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Protein A Chromatography was used to purify this SOCS3 antibody |
| Species React |
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Cat, Chinese Hamster, Cow, Dog, Elephant, Guinea Pig, Horse, Human, Mole Rat, Mouse, Pig, Rat, Rhesus Monkey, Sheep |
| Predicted React |
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Carolina anole, catfish, Chicken, Frog, New World monkey, Opossum, Panda |
| Host |
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Rabbit |
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Application
IHC (p): use antibody at 5 ug/ml
Storage
Store SOCS3 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.
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Background
SOCS3 is a member of the suppressor of cytokine signaling family with a molecular weight of ~ 25kDa. It is also an SSI or stat-induced stat inhibitor protein. IL-6, for example, induces SOCS3 expression and in turn SOCS3 negatively regulates IL-6 induced immune signaling and inflammatory pathways. SOCS3 is also induced by IL-10 and IFNgamma (1-3). SOCS3 plays an important role in the initial stages of B cell development and differentiation (1). IL-6, IL-10, and IFNgamma feedback loops and likely involvement of SOCS3 may also be critical in controlling and fine-tuning the inflammatory responses of activated macrophages through TLR ligands, a direct involvement therefore of SOCS3 in innate immune signaling pathways (4, 5). SOCS3 antibody provides an opportunity to identify and clarify the SOCS3 feedback control mechanism in host defense, innate immunity or autoimmune inflammatory cascades. |
Antigen
A portion of amino acids 170-220 from human SOCS3 was used as the immunogen for this antibody.
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Application Notes
The SOCS3 amino acid sequence used as immunogen for this antibody is 100% homologous in human, mouse, rat, cow, guinea pig, dog, chinese hamster, horse, mole rat, elephant, rhesus monkey, sheep, pig, and pole-cat, 94% in panda, carolina anole, new world monkey, chicken, wild turkey, opossum, and zebra finch, 89% in platypus, 72% in channel catfish, and 67% homologouus in frog. |
Genebank Info (Protein): NP_003946.3
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Gene ID: 9021
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Immunohistochemical analysis of SOCS3 in human brain probed with SOCS3 antibody at 5 ug/ml. |
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Reference
1. Jones, SA et al 2011 SOCS3 deletion in B cells alters cytokine response and germinal center output J. Immunol. 187: 6318-6326.
2. Alexander WS and DJ Hilton 2004 The role of suppressors of cytokine signaling (SOCS) protein in regulation of the immune response Ann., Rev. Immunol. 22: 503-529.
3. Croker, BA et al 2003 SOCS3 negatively regulates IL-6 signaling in vivo Nat. Immunol. 4: 540-546.
4. Bluyssen, HA et al 2010 IFN-gamma dependent SOCS3 expression inhibits IL-6 induced STAT3 phosphorylation and differentially affects IL-6 mediated transcriptional responses in endothelial cells. Am. J. Physiol. Cell. Physiol. 299: C354-367.
5. Hu, X SD Chakravarty and LB Ivashkiv 2008 Regulation of interferon and Toll-like receptor signaling during macrophage activation by opposing feedforward and feedback inhibition mechanisms Immunol. Rev. 226:41-56.
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