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| Monoclonal Antibody to Cytokeratin 19 (Clone BA17) |
| Catalog No |
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IMG-80041 |
| Contents |
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This antibody is supplied as tissue culture supernatant containing 0.05% sodium azide as preservative. |
| Isotype |
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Mouse IgG1 |
| Clone |
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BA17 |
| Species React |
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Human |
| Host |
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Mouse |
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| Application
IHC (paraffin): 1:25-1:50
IF/ICC:Users should optimize according to model and immunodetection system used (secondary reagents)
IP:Users should optimize according to model and immunodetection system used (secondary reagents)
Western blot analysis: Users should optimize ac
Storage
Store the antibody at 4-8°C.
Recommended Positive Control : Skin |
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Antigen
BALB/C mice were immunized with detergent insoluble extract of human mammary epithelium.
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Application Notes
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Specificity: This antibody recognizes keratin polypeptide of 40 kD. It is the smallest human keratin found in most simple and non- keratinizing stratified epithelia. This antigen is not present in adult intrafollicular epidermis. The antibody stains most of the epithelial cell types including ductal and glandular epithelia. Cellular Localization: Cytoplasmic. |
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Reference
1. Bartek et al. J Cell Sci 75: 17, 1985.
2. Bartek et al. Histochem J 18: 565, 1986.
3. Bartek et al. Int J Cancer 36: 299, 1985.
4. Bartek et al. J Cancer Clin Oncol 22: 1441, 1986
5. Yuan et al. Apoptosis 3: 161, 1998.
6. Zhou et al. J Biol Chem 274: 12861, 1999.
7. Eckert et al. Proc. Natl. Acad. Sci. 85: 1114, 1988.
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