Buffers & Reagents from IMGENEX: Mitochondrial Extract Kits
Mitochondrial Extraction Kit Cat. No. 10082K
The IMGENEX Mitochondrial Extraction Kit is a simple tool for isolation and enrichment of intact mitochondria. Based on the traditional Dounce homogenization method for tissue disruption and organelle isolation, the IMGENEX Mitochondrial Extraction Kit has been optimized to provide maximum enrichment, while minimizing mitochondrial damage. Easy to use and gentle on cells and tissue preparations, the fractionation kit combines an optimized protocol with gentle buffers to allow fast and thorough bench-top isolation of organelles. Quickly obtain the cellular fractions you need for your downstream applications.
IMGENEX now offers two kits enabling researchers to quickly fix and prepare cells for cell surface or intracellular staining and analysis.
Easy to use - kit contains all the necessary reagents
Fast - quickly isolate and enrich the mitochondrial fraction
Gentle - our optimized protocol and gentle buffers ensure maximum recovery of mitochondria
Flexible - can be used for a variety of different cell and tissue types
Mitochondrial Fractionation & Enrichment using 10082K
Mouse liver cells were fractionated using IMGENEX’s Mitochondrial Extraction kit. 20 ug of cytosolic (lane 1) and mitochondrial (lane 2) extracts were probed with anti-Cytochrome C antibody (IMG-101), and anti-Actin antibody (loading control, IMG-5142A). IMGENEX’s extraction kit efficiently separated the two fractions and no Cytochrome C (specific to mitochondria) was detected in the cytosolic extract.