Buffers and Reagents [Nuclear Extract Kits]

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Please contact your local IMGENEX distributor for pricing on these products. For a list of distributors, click here or go to www.imgenex.com/distributors.php

Buffers & Reagents from IMGENEX: Nuclear Extract Kits

Nuclear Fractionation Kit
Cat. No. 10081K

The IMGENEX Nuclear Extraction Kit provides a simple and convenient method for isolation and enrichment of nuclear and cytoplasmic extracts from mammalian cells and tissue samples. Based on the traditional Dounce homogenization method for tissue disruption and cellular fractionation, IMGENEX Nuclear Extraction Kit is easy to use, gentle on cells and tissue preparations, and perfect for applications requiring a high level of purity.

Benefits

Easy to use - kit contains all the necessary reagents
Fast - quickly generate nuclear and cytosolic fractions from your tissue or cell samples
Gentle - our optimized protocol and gentle buffers ensure quality results
Flexible - can be used for a variety of different cell and tissue types

Nuclear Fractionation &
Enrichment using 10081K

Figure 1. Western blot analysis of fractionated HeLa cells using IMG-5133A anti-Lamin B1. 20 ugs of HeLa cell protein were separated on 4-20% SDS-PAGE. Lane 1. Nuclear extract, Lane 2. Cytoplasmic extract, Lane 3. Total cell lysate. Western blot analysis using of Lamin B1 using IMG-5133A.

Figure 2. Western blot analysis of fractionated Cos-7 cells using IMG-359 anti-Histone H2B. Cos-7 cells were fractionated into Nuclei and Cytosol/ Membranes. ~15ug of each fraction was run on SDS PAGE. Western blot analysis of Histone H2b using IMG-359 in nuclear (lane 1) and cytosolic (lane 2) fractions.

Found 1, displaying 1 to 1

Cat.No	Description	Species	Unit	Price(USD)	MSDS
10081K	Nuclear Extraction Kit		1 kit	272.50

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