|
| Human Cervix Tissue Lysate Bundle Set, Cat. No. C004-003 |
|
| Cat No |
Description |
Units |
Price |
|
|
| C004-003 |
Human Cervix Tissue Lysate Bundle Set |
3 Vials |
-- |
|
|
|
| Tissue Bundled Set contains the following tumor & normal adjacent lysates (below). Lysates may also be purchased individually. |
|
| Extraction 1, Soluble Protein Fraction |
| C004-003T1 |
Human Cervix Tumor Tissue Lysate |
0.1 mg |
-- |
|
|
| C004-003TN1 |
Human Cervix Normal Tissue Lysate (matched) |
0.1 mg |
-- |
|
|
|
| Extraction 2, Insoluble Protein Fraction |
| C004-003T2 |
Human Cervix Tumor Tissue Lysate |
0.1 mg |
-- |
|
|
|
|
|
| Diagnosis: |
Squamous cell cervical carcinoma, moderately differentiated. |
Grade: 2 |
| Stage: |
I |
Sex: Female |
| TNM: |
T1bN0Mx |
Quantity: 100 |
| Age: |
54 |
|
| Concentration: |
1 mg/ml |
|
|
The vial is provided with a 10% overfill. Maximum
recovery can be obtained by centrifuging the vial briefly to collect any solution on the
cap and tube sides. |
|
| Storage: |
Aliquot single use volumes to avoid repeated
freeze/thaw cycles. From time of receipt, this product is stable for 12 months at
–70°C. |
|
| Lysate Preparation : |
Tissue specimens are homogenized in modified RIPA
buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further
extracted with a second buffer to obtain the less soluble protein fraction. The lysate
solution may appear turbid at cold temperatures due to insolubility of buffer components.
The solution should clear upon warming to room temperature. |
|
|
| Extraction 1: |
PBS, pH 7.4 |
1 µg/ml Aprotinin |
1 mM NaF |
| Modified RIPA Buffer: |
1 mM EDTA |
1 µg/ml Pepstatin-A |
0.1% SDS |
|
0.25% Na deoxycholate |
1 µg/ml Leupeptin |
1 mM PMSF |
|
1 mM Na3VO4 |
|
|
| Extraction 2: |
PBS, pH 7.4, 5.0 M Urea, 2.0 M Thiourea, 50mM DTT, 0.1% SDS |
|
| Usage: |
These lysates are proteomic discovery tools.
Researchers should validate and optimize for individual use. Potential applications MAY
include WB, immunoprecipitation, protein-protein interactions, ligand binding, ELISA. |
|
|
Note: For use in 1D and 2D gel electrophoresis,
the addition of a denaturing gel loading buffer with reducing agents may be required. |
|
| Warranty: |
The products sold hereunder are warranted only to
conform to the quantity and contents stated on the label at the time of delivery to the
customer. There are no warranties, expressed or implied, that extend beyond the
description on the label of the product. IMGENEX’s sole liability is limited to
either replacement of the products or refund of the purchase price. IMGENEX is not liable
for property damage, personal injury, or economic loss caused by the product. |
|
| Precautions: |
This material has tested negative for HbsAg, HIV
1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be
treated as a potentially infectious agent and disposed of appropriately. |
|
