Expression Systems

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Expression Systems

Retroviruses are very efficient tools for delivering genes into dividing cells. The RetroMax™ Retroviral Expression vector system is based on the pCL vector system and has been designed to maximize recombinant–retrovirus titers in a simple, efficient, and flexible experimental system1. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal and are derived from safety- modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome. Thee RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.

The following Expression Systems are available:

Found 13, displaying 1 to 13

Cat.No	Description	Species	Unit	Price(USD)	MSDS
10054K	Beta-galactosidase quantitative kit. It contains all the reagents necessary for detection of B-galactosidase enzyme in cells and tissues. This kit utilizes CPRG as substrate.		1 kit	N/A
10049P	Foxp3, human (full length cDNA) in PCLXSN Retroviral expression vector		1 µg	N/A
10042P	pCLNCX Expression Vector		10 µg	N/A
10053K	The B-galactosidase staining kit contains all the reagents necessary for detection of B-galactosidase enzyme in cells and tissues. This kit utilizes X-gal as substrate.		1 kit	N/A
10040K	The complete RetroMax System includes 4 pCL expression plasmids, 3 packaging plasmids, one reporter plasmid, transfection reagents, and manual.		1 kit	N/A
10047P	The pCL-10A1 packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect most mammalian cells including hamster cells.Sequence information for this plasmid is not available.		10 µg	N/A
10046P	The pCL-Ampho packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect most mammalian cells, but not hamster cells. Sequence information for this plasmid is not available.		10 µg	N/A
10045P	The pCL-Eco packaging vector is a part of the RetroMax expression system. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.Sequence information for this plasmid is not available.		10 µg	N/A
10048P	The pCL-MFG-LacZ reporter vector is a part of the RetroMax expression system. It contains a full-length bacterial b-galactosidase (lacZ) under the control of the murine LTR. Sequence information for this plasmid is not available.		10 µg	N/A
10044P	The pCLNDX expresion vector is a part of the RetroMax expression system. The cloned genes are under the control of DHFR promoter.		10 µg	N/A
10043P	The pCLNRX expression vector is a part of the RetroMax expression system. The cloned genes are under the control of RSV promoter.		10 µg	N/A
10041P	The pCLXSN vector is a part of the RetroMax expression system.		10 µg	N/A
10041K	This RetroMax System includes four pCL expression plasmids.		1 kit	N/A

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