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I am unable to get IMG-6135A (Anti-S1P2 Edge5) to work. I am able to see a lot of signals but no signal that is obviously due to the presence of S1P2. Most of the signals occurred in RIPA lysates of human primary Granulosa cells, but not in the positive control.
Answer :
Thank you for your inquiry about IMG-6135A http://imgenex.com/antibody_details.php?catalog=IMG-6135A We test this antibody in heart. From you note, it is unclear what you used as a positive control. Did you use heart? If you did not, please use heart as a positive control. We have not tested the antibody in granulosa cells and don't have information about the banding patterns in granulosa cells.