A reporter gene assay is a common and widely utilized molecular technique for measurement of the activity of promoters.   The ability to monitor the regulatory activity of a target through its DNA sequence is often designed as follows:  A target promoter sequence or response element is linked to a detectable reporter gene which encodes an enzyme like SEAP (secreted alkaline phosphatase),  a fluorescent protein such as GFP, or a luciferase such as Firefly luciferase or Renilla luciferase, allow relative and/or specific contribution of target gene participation as a regulatory element in the assay.   Because of their speed, dynamic range and sensitivity and use of relatively inexpensive ELISA, Luminescent or Fluorescent read-out instruments,  the SEAP and Luciferase assay provide invaluable tools for the studies of regulatory pathways. The LightSwitch™ Luciferase Assay Reagent enables one-step reagent addition to measure luciferase signal. The luciferase assay can be used to measure the improved luminescent reporter gene (RenSP) found in IMGENEX's line of LUCPorter stable reporter cell lines. 

The LightSwitch™ Luciferase Assay Reagent and assay-related products and stable cell lines can be found here

IMGENEX is an authorized seller of LightSwitch™, SwitchGear's Luciferase Assay Reagent.