| Recombinant Human Bone Morphogenetic Protein -2 (BMP-2) |
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Description |
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Recombinant Human Bone Morphogenetic protein-2 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. BMP-2 is purified by proprietary chromatographic techniques. |
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Physical Appearance |
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Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
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BMP-2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5. |
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Solubility |
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It is recommended to reconstitute the lyophilized rhBMP-2 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions |
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Stability |
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Lyophilized BMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
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Purity |
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Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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Dimers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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IMGENEX's BMP-2 is fully biologically active when compared to standard.The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml. |
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Endotoxin |
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Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-2 . |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name:BMP2 Synonyms:BMP2A |
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Protein synonyms/aliases |
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Bone morphogenetic protein 2 precursor (BMP-2) (BMP-2A). |
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Protein Family |
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Belongs to the TGF-beta family. |
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Precursor Domain architecture |
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<IMG border=0 SRC=http://www.ebi.ac.uk/interpro/matchline?code=AP12643,C,<div> |
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Protein links |
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Reference
1. Histological analysis of a displaced femoral ring allograft spacer filled with a recombinant human bone morphogenetic protein-2-soaked collagen sponge. A case report.
J Bone Joint Surg Am 2005 Oct;87(10):2318-22
2. Expression of bone morphogenetic protein 2 in normal spontaneous labor at term, preterm labor, and preterm premature rupture of membranes.
Am J Obstet Gynecol 2005 Sep;193(3 Pt 2):1137-43
3. Recombinant human bone morphogenetic protein-2: a novel osteoinductive alternative to autogenous bone graft?
Acta Orthop Belg 2005 Apr;71(2):133-48
4. Murine spinal fusion induced by engineered mesenchymal stem cells that conditionally express bone morphogenetic protein-2.
J Neurosurg Spine 2005 Jul;3(1):47-52
5. Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells.
J Biomed Mater Res A 2005 Oct 1;75(1):210-5
6. Tissue engineering with recombinant human bone morphogenetic protein-2 for alveolar augmentation and oral implant osseointegration: experimental observations and clinical perspectives.
Clin Implant Dent Relat Res 2005;7(2):112-9
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| Research purposes only.
Not for diagnostic or in vivo use. |
