| Recombinant Human Ciliary Neurotrophic Factor (CNTF) |
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Description |
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Recombinant Human CNTF produced in E. Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22706 Daltons. Recombinant CNTF is purified by proprietary chromatographic techniques. |
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Physical Appearance |
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Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
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Lyophilized from a concentrated (1mg/ml) solution in water containing 5mM sodium Phosphate buffer pH=7.5 and 5mM sodium chloride. |
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Solubility |
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It is recommended to reconstitute the lyophilized CNTF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
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Stability |
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Lyophilized CNTF, although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution CNTF should be stored at 4 C between 2-7 days and for future use, below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
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Purity |
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE silver stained gel. |
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Dimers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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Imgenex's Recombinant Human Ciliary Neurotrophic Factor is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5 x 10 IU/mg. |
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Endotoxin |
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Less than 0.1 ng/µg (IEU/µg) of recombinant CNTF. |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name:CNTF |
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Protein synonyms/aliases |
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Ciliary neurotrophic factor (CNTF). |
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Protein Family |
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Belongs to the CNTF family. |
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Precursor Domain architecture |
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Protein links |
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Reference
1. Relation of weight maintenance and dietary restraint to peroxisome proliferator-activated receptor gamma2, glucocorticoid receptor, and ciliary neurotrophic factor polymorphisms.
Am J Clin Nutr 2005 Oct;82(4):740-6
2. Ciliary neurotrophic factor-immunoreactivity in olfactory sensory neurons.
Neuroscience 2005;134(4):1179-94
3. Ciliary neurotrophic factor promotes muller glia differentiation from the postnatal retinal progenitor pool.
Dev Neurosci 2004;26(5-6):359-70
4. Dedifferentiation of adult human myoblasts induced by ciliary neurotrophic factor in vitro.
Mol Biol Cell 2005 Jul;16(7):3140-51
5. Rapid protein kinase C-dependent reduction of rat skeletal muscle voltage-gated sodium channels by ciliary neurotrophic factor.
J Physiol 2005 Jun 15;565(Pt 3):827-41
6. E xpression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats.
Neurosci Lett 2005 Apr 4;377(3):170-5
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| Research purposes only.
Not for diagnostic or in vivo use. |
