| Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor |
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Description |
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Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. Recombinant GM-CSF is purified by proprietary chromatographic techniques. |
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Physical Appearance |
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Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
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GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4±0.1. |
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Solubility |
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It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
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Stability |
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Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccatedbelow -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future usebelow -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
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Purity |
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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Dimers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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Imgenex's GM-CSF is fully biologically active when compared to standard.The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1 ng/ml, corresponding to a Specific Activity of 11.1x10 6IU/mg. |
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Endotoxin |
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Less than 0.1 ng/µg (IEU/µg) of GM-CSF . |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name:CSF2 Synonyms:GMCSF |
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Protein synonyms/aliases |
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Granulocyte-macrophage colony-stimulating factor precursor (GM-CSF) (Colony-stimulating factor) (CSF) (Sargramostim) (Molgramostin). |
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Protein Family |
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Belongs to the GM-CSF family. |
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Precursor Domain architecture |
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<IMG border=0 SRC=http://www.ebi.ac.uk/interpro/matchline?code=AP04141,C,<div> |
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Protein links |
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Reference
1. Adenosine potentiates stimulatory effects on granulocyte-macrophage hematopoietic progenitor cells in vitro of interleukin-3 and stem cell factor, but not those of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor and interleukin-11.
Physiol Res 2005 Dec 12;
2. Acute CD4+ T lymphocyte-dependent interleukin-1-driven arthritis selectively requires interleukin-2 and interleukin-4, joint macrophages, granulocyte-macrophage colony-stimulating factor, interleukin-6, and leukemia inhibitory factor.
Arthritis Rheum 2005 Dec;52(12):3749-3754
3. Granulocyte-macrophage colony-stimulating factor as an arteriogenic factor in the treatment of ischaemic stroke.
Expert Opin Biol Ther 2005 Dec;5(12):1547-56
4. [The pulmonary expression of granulocyte-macrophage colony-stimulating factor and surfactant protein in adult idiopathic pulmonary alveolar proteinosis.]
Zhonghua Nei Ke Za Zhi 2005 Nov;44(11):832-5
5. A vesicular stomatitis virus recombinant expressing granulocyte-macrophage colony-stimulating factor induces enhanced T-cell responses and is highly attenuated for replication in animals.
J Virol 2005 Dec;79(24):15043-53
6. Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts: a placebo controlled randomized study in healthy subjects.
Eur J Med Res 2005 Oct 18;10(10):411-8
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| Research purposes only.
Not for diagnostic or in vivo use. |
