| Recombinant HIV-I Envelope (spanning C-terminus of gp120 and gp41) |
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Description |
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This antigen is an E. coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a the C-terminus of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and doesn’t represent a linear HIV-I envelope sequence. rHIV-I is a non-glycosylated, 233 aa polypeptide chain, having a molecular mass of 27275.88, pI=9.68. |
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Physical Appearance |
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Sterile filtered colorless clear solution |
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Formulation |
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The antigen is dissolved in 0.5X PBS containing 6M urea. |
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Stability |
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Store at -20°C, stable for five years when kept frozen. Stable for 1 month at 4°C. Store under sterile conditions, avoid repeated freeze/thaws. |
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Purity |
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Greater than 98.0% as determined by HPLC analysis |
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Dimers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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The recombinant HIV-1 envelope protein was thoroughly tested in various tests as well as ELISA using the standard BBI serum panels.
All antibody detection tests on recombinant HIV-1 envelope protein were as sensitive or more than the standard Abott third generation tests.
The recombinant HIV-1 Envelope protein has been shown to be immunoreactive with all sera of HIV-1 and HIV-type O infected individuals tested, and with 60-80% of HIV-2 infected individuals.
The recombinant HIV-1 Envelope protein is suitable for both ELISA and western blot analysis. |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name: env |
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Protein synonyms/aliases |
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Envelope glycoprotein gp160 precursor (Env polyprotein) |
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Protein Family |
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Precursor Domain architecture |
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Protein links |
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| Research purposes only.
Not for diagnostic or in vivo use. |
