Recombinant Human Insulin produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Recombinant Insulin is purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Recombinant Human Insulin was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility
It is recommended to reconstitute the lyophilized recombinant Insulin in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Recombinant Human Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Insulin should be stored at 4 C between 2-7 days and for future use below -18 C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and Aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity
IMGENEX's recombinant Insulin is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
Endotoxin
Less than 0.1 ng/µg (IEU/µg) of recombinant Insulin.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Insulin as a Reference Standard.
Usage
This material is offered by Imgenex for research, laboratory or further evaluation purposes.
