Recombinant Human Interferon-alpha 2b (IFN-a)

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Recombinant Human Interferon-alpha 2b (IFN-a)

Cat.No
IMR-237

Description
Interferon-alpha 2b

Unit
20 µg

Price(USD)
152.60

MSDS
N/A

Catalog No	:	IMR-237
Formulation	:	Lyophilized from a (1mg/ml) solution containing 2.3mg Sodium phosphate dibasic and 0.55mg sodium phosphate monobasic buffer.

Description

Recombinant Human Interferon-alpha 2b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. The Interferon-alpha 2b gene was obtained from human leukocytes. IFN-alpha 2b is purified by proprietary chromatographic techniques.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a (1mg/ml) solution containing 2.3mg Sodium phosphate dibasic and 0.55mg sodium phosphate monobasic buffer.

Solubility

It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Interferon-alpha 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Interferon-alpha 2b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been completely removed enzymatically.

Dimmers and Aggregates

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity

Imgenex's IFN-alpha 2b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6 x 108 IU/ mg.

Endotoxin

Less than 0.1 ng/µg (IEU/µg) of Recombinant Interferon-alpha 2b.

Protein Content

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Interferon-alpha 2b as a Reference Standard.

Usage

This material is offered by Imgenex for research, laboratory or further evaluation purposes.

Gene

Name: IFNA8

Protein synonyms/aliases

Interferon alpha-8 precursor (Interferon alpha-B2) (Interferon alpha-B) (LeIF B).

Protein Family

Belongs to the alpha/beta interferon family.

Precursor Domain architecture

Protein links