| Recombinant Human Interleukin-1 receptor antagonist (IL-1ra) |
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Cat.No
IMR-245 |
Description
Interleukin-1 receptor antagonist (IL1RA)
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Unit
20 µg |
Price(USD)
457.80 |
MSDS
N/A |
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| Catalog No |
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IMR-245 |
| Formulation |
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Lyophilized protein with no additives. |
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Description |
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Recombinant Human IL-1ra produced in E.Coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton. Recombinant IL-1ra is purified by proprietary chromatographic techniques. |
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Physical Appearance |
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Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
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Lyophilized protein with no additives. |
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Solubility |
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It is recommended to reconstitute the lyophilized IL-1ra in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
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Stability |
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Lyophilized IL-1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-1ra should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
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Purity |
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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Dimmers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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IMGENEX's Interleukin-1ra is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml. |
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Endotoxin |
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Less than 0.1 ng/µg (IEU/µg) of IL-1ra. |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1ra as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name: IL1RN Synonyms :IL1F3, IL1RA |
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Protein synonyms/aliases |
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Interleukin-1 receptor antagonist protein precursor (IL-1ra) (IRAP) (IL1 inhibitor) (IL-1RN) (ICIL-1RA). |
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Protein Family |
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Belongs to the IL-1 family. |
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Precursor Domain architecture |
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<IMG border=0 SRC=http://www.ebi.ac.uk/interpro/matchline?code=AP18510,C,<div> |
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Protein links |
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Reference
1. [Effects of immunoglobulin on the neuronal expression of IL-1beta and IL-1ra and the neuronal death at hippocampus in rats with convulsion induced by pentylenetetrazol.]
Zhonghua Er Ke Za Zhi 2005 Nov;43(11):849-51
2. Updated consensus statement on biological agents, specifically tumour necrosis factor {alpha} (TNF{alpha}) blocking agents and interleukin-1 receptor antagonist (IL-1ra), for the treatment of rheumatic diseases, 2005.
Ann Rheum Dis 2005 Nov;64 Suppl 4:iv2-14
3. Ischemic preconditioning modulates the expression of several genes, leading to the overproduction of IL-1Ra, iNOS, and Bcl-2 in a human model of liver ischemia-reperfusion.
FASEB J 2005 Oct;19(12):1617-26
4. [Interleukin-1 (IL-1) alpha, beta, IL-1 receptor, IL-1 receptor antagonist (IL-1ra)]
Nippon Rinsho 2005 Aug;63 Suppl 8:60-4
5. IL-1 receptor antagonist (IL-1Ra) gene polymorphism in patients with inflammatory bowel disease in India.
Scand J Gastroenterol 2005 Jul;40(7):827-31
6. Association of IL-1Ra gene polymorphism, but no association of IL-1beta and IL-4 gene polymorphisms, with Kawasaki disease.
J Clin Lab Anal 2005;19(3):99-102
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| Research purposes only.
Not for diagnostic or in vivo use. |
