| Recombinant Human Interleukin-3 (IL-3) |
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Cat.No
IMR-249 |
Description
Interleukin-3
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Unit
2 µg |
Price(USD)
152.60 |
MSDS
N/A |
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| Catalog No |
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IMR-249 |
| Formulation |
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Lyophilized from a concentrated (1mg/ml) solution in water containing5mg HSA and 4.3mg Sodium Chloride. |
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Description |
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Recombinant Human IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-3 is purified by proprietary chromatographic techniques. |
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Physical Appearance |
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Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
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Lyophilized from a concentrated (1mg/ml) solution in water containing5mg HSA and 4.3mg Sodium Chloride. |
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Solubility |
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It is recommended to reconstitute the lyophilized rHuIL-3 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
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Stability |
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Lyophilized rHuIL-3 although stable at room temperature for 3 weeks, should be stored desiccatedbelow -18 C. Upon reconstitution rHuIL-3 should be stored at 4 C between 2-7 days and for future usebelow -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
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Purity |
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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Dimmers and Aggregates |
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Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
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Biological Activity |
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IMGENEX's rHuIL-3 is fully biologically active when compared to standard.The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1 x 10 IU/mg. |
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Endotoxin |
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Less than 0.1 ng/µg (IEU/µg) of rHuIL-3. |
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Protein Content |
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Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard. |
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Usage |
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This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
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Gene |
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Name:IL3 |
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Protein synonyms/aliases |
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Interleukin-3 precursor (IL-3) (Multipotential colony-stimulatingfactor) (Hematopoietic growth factor) (P-cell-stimulating factor) (Mast-cell growth factor) (MCGF). |
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Protein Family |
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Belongs to the IL-3 family. |
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Precursor Domain architecture |
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<IMG border=0 SRC=http://www.ebi.ac.uk/interpro/matchline?code=AP08700,C,<div> |
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Protein links |
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Reference
1. Diphtheria toxin fused to variant human interleukin-3 induces cytotoxicity of blasts from patients with acute myeloid leukemia according to the level of interleukin-3 receptor expression.
Blood 2005 Oct 1;106(7):2527-9
2. Toxicology and pharmacokinetics of DT388IL3, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human interleukin 3 (IL3), in cynomolgus monkeys.
Leuk Lymphoma 2004 Aug;45(8):1647-56
3. [Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy gamma-rays]
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2004 Jan;16(1):22-5
4. Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system.
Protein Expr Purif 2003 Sep;31(1):34-41
5. Recombinant human interleukin-3 administered concomitantly with chemotherapy in patients with relapsed small cell lung cancer.
J Exp Ther Oncol 2002 Jan-Feb;2(1):47-52
6. Evidence for a lectin activity for human interleukin 3 and modeling of its carbohydrate recognition domain.
J Biol Chem 2002 Oct 11;277(41):38764-71
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| Research purposes only.
Not for diagnostic or in vivo use. |
