|
| |
|
|
|
|
|
 |
Description |
|
Recombinant Human PTH produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 34 amino acids and having a molecular mass of 4117.8 Dalton. rHuPTH is purified by proprietary chromatographic techniques. |
|
|
Physical Appearance |
|
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
|
Formulation |
|
The protein (1mg/ml) was lyophilized after extensive dialyses against 1.15 mg sodium citrate, sodium chloride 7.31mg, 0.21mg citric acid, 0.1117 EDTA-Na2, 0.2mg Tween 80 and 50mgs Mannitol. |
|
|
Solubility |
|
It is recommended to reconstitute the lyophilized rHuPTH in sterile 18MÙ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
|
|
Stability |
|
Lyophilized rHuPTH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuPTH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
|
|
Purity |
|
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. |
|
|
Amino Acid Sequence |
|
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Val-Ser-Glu-Ile. |
|
|
Dimers and Aggregates |
|
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
|
|
Biological Activity |
|
Imgenex's rHuPTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity o f1.0 x 104 Units/mg. |
|
|
Endotoxin |
|
Less than 0.1 ng/µg (IEU/µg) of rHuPTH. |
|
|
Protein Content |
|
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm. Analysis by RP-HPLC, using a calibrated solution of PTH as a Reference Standard. |
|
|
Usage |
|
This material is offered by Imgenex for research, laboratory or further evaluation purposes. |
|
|
Gene |
|
Name:PTH |
|
|
Protein synonyms/aliases |
|
Parathyroid hormone precursor (Parathyrin) (PTH) (Parathormone). |
|
|
Protein Family |
|
Belongs to the parathyroid hormone family. |
|
|
Precursor Domain architecture |
|
<IMG border=0 SRC=http://www.ebi.ac.uk/interpro/matchline?code=AP01270,C,<div> |
|
|
Protein links |
|
  |
|
|
|
|
|
Reference
1. Canine hyperadrenocorticism: effects of trilostane on parathyroid hormone, calcium and phosphate concentrations.
J Small Anim Pract 2005 Nov;46(11):537-42
2. Relationship between serum parathyroid hormone levels, vitamin D sufficiency, and calcium intake.
JAMA 2005 Nov 9;294(18):2336-41
3. Intraoperative parathyroid hormone testing improves cure rates in patients undergoing minimally invasive parathyroidectomy.
Surgery 2005 Oct;138(4):583-7; discussion 587-90
4. [Parathyroid hormone for osteroporsis: why and who to treat?]
Rev Med Suisse 2005 Oct 5;1(35):2272-4 , 2276-7
5. Parathyroid hormone and biochemical profile in chronic kidney disease patients in South India.
Hemodial Int 2005 Jan;9(1):63-7
6. Relationship between parathyroid calcium-sensing receptor expression and potency of the calcimimetic, cinacalcet, in suppressing parathyroid hormone secretion in an in vivo murine model of primary hyperparathyroidism.
Eur J Endocrinol 2005 Oct;153(4):587-94
|
|
|
|
|
| Research purposes only.
Not for diagnostic or in vivo use. |
