Epidermal growth factor (EGF) is a neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. Recombinant human Epidermal Growth Factor produced in E. coli is a single, non-glycosylated polypeptide chain containing 53 amino acids and having a molecular mass of 6,222 Daltons.
Physical Appearance
Sterile Filtered; white lyophilized (freeze-dried) powder.
Formulation
100 ug of lyophilized protein
Stability
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity
Purity greater than 95% as determined by: Analysis by RP-HPLC, reducing and non-reducing SDS-PAGE.
Recombinant human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells, is 20.25 pg/ml.
Endotoxin
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content
Protein content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Research purposes only.
Not for diagnostic or in vivo use.
