Recombinant mouse Epidermal Growth Factor (EGF) protein
Catalog No
:
IMR-293-0100
Description
Recombinant mouse Epidermal Growth Factor has an effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Recombinant mouse Epidermal Growth Factor produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.
Physical Appearance
Sterile Filtered; white lyophilized (freeze-dried) powder.
Formulation
100 ug lyophilized protein
Stability
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity
Purity greater than 97% as determined by: Reducing and non-reducing SDS-PAGE and analytical HPLC.
Recombinant mouse EGF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.02 ng/ml.
Endotoxin
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content
Protein content determined by: UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
Research purposes only.
Not for diagnostic or in vivo use.
