| Recombinant mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) protein |
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Catalog No |
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IMR-285-0005 |
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Description |
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M-GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL-2 -beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant mouse GM- CSF produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14,285.35 Daltons. |
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Physical Appearance |
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Sterile Filtered; white lyophilized (freeze-dried) powder. |
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Formulation |
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5 ug of protein lyophilized from 10 mM acetic acid. |
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Stability |
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Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
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Purity |
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Purity greater than 95% as determined by: Analysis by RP-HPLC, reducing and non- reducing SDS-PAGE. |
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Amino Acid Sequence |
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MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK |
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Biological Activity |
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Mouse GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse FDC-P1 cell line is 0.26 ng/ml. |
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Endotoxin |
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Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
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Protein Content |
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Protein content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
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| Research purposes only.
Not for diagnostic or in vivo use. |
