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Polyclonal Antibody to DEFA6 |
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Cat.No
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Description
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Format
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Unit
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Price(USD)
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MSDS
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| Catalog No: |
IMX-6765 |
| Contents: |
100 ul; crude DEFA6 rabbit anti-serum with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Species: |
Chimpanzee, Human |
| Predicted React: |
New World monkey, Orangutan, Rhesus Monkey, White-cheeked gibbon |
| Host: |
Rabbit |
| Application: |
Peptide ELISA: 1:100-1:1000 |
| Storage: |
Store DEFA6 antibody at -20°C. |
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Background
Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. IMGENEX is pleased to be able to offer researchers access to cutting-edge products that are still in the process of characterization/validation. IMGENEX’s ‘IMX’ antibody line is shown by ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest.
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We cannot guarantee that the yet-to-be-fully validated antibody will work in any application other than in an ELISA against the peptide used as immunogen and therefore cannot offer a refund if the antibody does not work in your application. |
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The antibody is offered at a lower price compared to a more highly validated antibody. |
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We are not able to provide the immunogen for the antibodies, but do provide the amino acid sequence. |
These antibodies are not fully validated and we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.
DEFA6, also known as Defensin-6, is an 11kDa protein and member of the alpha-defensin grouping. Alpha-defensins are 29-42 amino acid peptides with documented innate anti-microbial activity (1).In humans, six alpha-defensins have been identified –four called Human Neutrophil Peptides (HNP) 1, 2, 3 and 4, are part of neutrophil defense mechanisms in systemic innate immunity. Human Defensin (HD) 5 and 6 are expressed in intestinal Paneth cells (2), and contribute to normal gut host defense and are dramatically increased in chronically inflamed colon (3). Paneth cells especially express DEFA6 in their lysozyme granules. Because of alpha-defensin expression in chronically inflamed gut tissue, it has also been determined and proposed that DEFA6 has potential as a marker for colon cancer (4). By RT-PCR analysis it has been determined that the highest expression of DEFA6 is found in adenoma tissue vs normal colon tissue –over 100 fold increase whereas less fold increase and frequency in carcinoma tissue overall vs normal tissue. DEFA6 greatly elevated expression patterns in adenoma vs carcinoma tissue is proposed to have more value as an indicator to discriminate between pre-malignant or earlier colorectal cancer or staging of colon cancer vs colon cancer overall (4). |
Antigen
Amino acids 28-45 [PLQAKAYEADAQEQRGAN] of human DEFA6 were used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, and chimpanzee, 83% in rhesus monkey, and white-cheeked gibbon, 78% in orangutan, and 61% homolgous in new world monkey. |
Genebank Info (Protein)
NP_001917.1 |
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Reference
1. Radeva, MY et al 2010 BMC Cancer 10: 588-593.
2. Menendez, A and BB Finlay 2007 Curr Opin. Immunol. 19: 385-391.
3. Cunliffe, RN 2003 Mol. Immunol 40: 463-467.
4. Cunliffe, RN and YR Mahida 2004 J. Leuk. Biol. 75: 49-58.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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