Monoclonal Antibody to IL-1b (Clone IMG43N8F6)

Background
IL-1b (Interleukin-1 beta, IL-1beta) is a potent inflammatory cytokine which has been well studied.  Produced as a precursor, especially in myeloid cells and monocytes/macrophages, IL-1b is processed by cleavage with caspase-1, or ICE, from the 31 kD inactive precursor to the 18kD active mature form secreted from cells as an end-result of the inflammasome complex and activation sequence (1, 2).  In response to various TLR ligands, myeloid cells or macrophages activate NLRP3 inflammasomes and secrete IL-1b.  The rate of IL-1b processing has been correlated to redox state or levels of ROS generated (3, 4).  IL-1b is directly involved in a variety of diseases -–auto-inflammatory diseases, acute inflammatory attacks such as ischemic injury in myocardial infarcts or in chronic inflammatory processes of atherosclerosis or Type 2 diabetes (5, 6).  The ability to detect and quantitate active IL-1b is critical for understanding how to regulate its activity as a therapeutic target.

Antigen
Full-length recombinant human IL-1b protein was used as immunogen for this antibody.

Application Notes
View additional IL-1 beta antibodies and products here

Genebank Info (Protein)
NP_000567

Reference
1. Dinarello, CA 2011 A clinical perspective of IL-1b as the gatekeeper of inflammation.  Eur. J. Immunol. 41: 1203-1207
2. Hanamsagar, R, V Torres and T Kielian 2011  Inflmmasome activation and IL-1b/IL-18 processing are influenced by distinct pathways in microglia. J. Neurochem. 119: 736-748
3. Tassi, S et al 2009 Pathogen-induced interleukin-1beta processing and secretion is regulated by a biphasic redox response. J. Immunol. 183: 1456-1462.
4. Gu, Y et al 1997  Activation of interferon –gamma inducing factor mediated by interleukin-1 converting enzyme  Science 275: 206-209
5. Carta, S et al 2011  The rate of production of interleukin-1b Secretion in different myeloid cells varies with the extent of Redox Response to Toll-like Receptor triggering.  J. Biol. Chem. 286: 27069-27080
6. Ghayur, T et al 1997 Caspases-1 Processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production Nature 386: 619-623
7. Koustav Ganguly, Swapna Upadhyay, Martin Irmler, Shinji Takenaka, Katrin Pukelsheim, Johannes Beckers, Eckard Hamelmann, Holger Schulz, Tobias Stoeger. Pathway focused protein profiling indicates differential function for IL-1B, -18 and VEGF during initiation and resolution of lung inflammation evoked by carbon nanoparticle exposure in mice. Part Fibre Toxicol. 2009; 6: 31.
8. Isabelle Jeru, Véronique Hentgen, Sylvain Normand, Philippe Duquesnoy, Emmanuelle Cochet, Adriana Delwail, Gilles Grateau, Sandrine Marlin, Serge Amselem, Jean-Claude Lecron. Role of IL-1b in NLRP12-associated autoinflammatory disorders and resistance to anti-IL-1 therapy. Pediatr Rheumatol Online J. 2011; 9(Suppl 1): O31.
9. Brett D. Hollingshead, Timothy V. Beischlag, Brett C. DiNatale, Preeti Ramadoss, Gary H. Perdew. Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells. Cancer Res. 2008 May 15; 68(10): 3609–3617.
10. G Murphy, J Thornton, R McManus, N Swan, B Ryan, CA O’Morain, DJ Hughes, M O’Sullivan. Association of gastric disease with polymorphisms in the inflammatory related genes IL-1B, IL-1RN, IL-10, TNF and TLR4. Eur J Gastroenterol Hepatol. 2009 June; 21(6): 630–635.