Background A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats. |
Reference
1. Herzenberg LA, Parks D, Sahaf B, Perez O, Roederer M, Herzenberg LA. The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford. Clin Chem. 2002 Oct;48(10):1819-27. 2. Loken MR, Parks DR, Herzenberg LA. Two-color immunofluorescence using a fluorescence-activated cell sorter. J Histochem Cytochem. 1977 Jul;25(7):899-907. 3. Ibrahim SF, van den Engh G. Flow cytometry and cell sorting. Adv Biochem Eng Biotechnol. 2007;106:19-39. 4. Nicole Baumgartha, Mario Roedererb. A practical approach to multicolor flow cytometry for immunophenotyping. Journal of Immunological Methods 243 (2000) 77–97. 5. Jonathan G. Crowston, Lydia H. Chang, Peter H. Constable, Julie T. Daniels, Arne N. Akbar and Peng T. Khaw. Apoptosis Gene Expression and Death Receptor Signaling in Mitomycin-C–Treated Human Tenon Capsule Fibroblasts. Invest. Ophthalmol. Vis. Sci. March 2002 vol. 43 no. 3 692-699. 6. Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry. 2001 Nov 1;45(3):194-205. |