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Product : Antibody >> Alexa Fluor Antibodies , Isotype Controls >> Mouse IgG2b Isotype Control
      
 

Mouse IgG2b Alexa Fluor® 647 Conjugate Isotype Control

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
20111AF647
Mouse IgG2b Isotype Control Alexa Fluor® 647
0.1 mg
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Catalog No: 20111AF647
Contents: 0.1 mg of mouse isotype control in 0.2 ml of PBS containing 0.05% BSA and 0.05% sodium azide. 
Isotype: Mouse IgG2b
Clone: MPC-11
Host: Mouse
Application: There are multiple applications that utilize mouse isotype control antibodies including flow cytometry, IHC and IF.
Storage: Store mouse isotype control at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
 

Background
A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.

Application Notes
The MPC-11 hybridoma cell line was grown and tissue culture supernatant was purified using affinity chromatography.

Click here to see a list of additional mouse isotype control antibodies.
 
Mouse IgG2b Isotype Control Antibody

Cell surface flow analysis of CD86 in human monocytes using 0.1 ug of IMG-6548AF647. The shaded histogram represents human monocytes alone; green represents mouse isotype control (20111AF647); red represents CD86 IMG-6548AF647 antibody.

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Reference
1. Herzenberg LA, Parks D, Sahaf B, Perez O, Roederer M, Herzenberg LA.  The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford.  Clin Chem. 2002 Oct;48(10):1819-27.
2. Loken MR, Parks DR, Herzenberg LA. Two-color immunofluorescence using a fluorescence-activated cell sorter. J Histochem Cytochem. 1977 Jul;25(7):899-907.
3. Ibrahim SF, van den Engh G. Flow cytometry and cell sorting. Adv Biochem Eng Biotechnol. 2007;106:19-39.
4. Nicole Baumgartha, Mario Roedererb.  A practical approach to multicolor flow cytometry for immunophenotyping. Journal of Immunological Methods 243 (2000) 77–97.
5. Jonathan G. Crowston, Lydia H. Chang, Peter H. Constable, Julie T. Daniels, Arne N. Akbar and Peng T. Khaw. Apoptosis Gene Expression and Death Receptor Signaling in Mitomycin-C–Treated Human Tenon Capsule Fibroblasts. Invest. Ophthalmol. Vis. Sci. March 2002 vol. 43 no. 3 692-699.
6. Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry. 2001 Nov 1;45(3):194-205.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.

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