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Polyclonal Antibody to GNLY |
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| Catalog No: |
IMG-6813A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography was used to purify this antibody |
| Species: |
Chimpanzee, Human |
| Predicted React: |
Orangutan |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 3-5 ug/ml |
| Storage: |
Store GNLY antibody at 4oC, stable for 6 months. For long-term storage, aliquot and store antibody at -20oC. |
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Background
GNLY (Granulysin) is characterized as a pro- inflammatory, cytolytic, 9kDa or 15kDa peptide found in granular endosomal compartments of NK cells and cytotoxic T cells (1). GNLY's cytolytic activity, in part, has been attributed to induction of cathepsin B release from target cell lysosomes which leads a damaging barrage of mitochondrial targets and a subsequent rapid cell lysis or necroptosis (2). Pro-inflammatory activity of GNLY is exhibited by induced and increased expression of inflammatory and chemoattractant cytokines such as RANTES, MCP-1 IL-1 and IL-6 on Tcells, monocytes and other cells (1, 3). Induction of CD80 and CD86 on DCs and their production of IL-6 and TNFa also illustrate its activity as a functional enhancer of the DC-Tcell synapse driving and polarizing specific Tcell responses (3). More recently, the finding that GNLY stimulation of Dendritic Cells is integrated with TLR4 and MyD88 illustrates a newly described role as an alarmin and part of the Innate Immune Response in addition to original cytolytic and adjuvant inflammatory activity (4). Antibodies to detect GNLY are valuable for assessment of its role in the varied areas of innate immunity, inflammation, chemo-attraction and cytolytic activity. |
Antigen
A portion of amino acids 70-120 from human GNLY was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for this GNLY antibody is 100% homologous in human, and chimpanzee, and 81% homologous in orangutan. |
Genebank Info (Protein)
NP_006424.2 |
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Western blot analysis of GNLY using GNLY antibody. HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. IMGENEX's goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Krensky AM and C Clayberger 2009 Biology and Clinical relevance of Granulysin Tissueigens 73: 193-198.
2. Tewary P 2010 Granulysin activates antigen-presenting cells through TLR4 and acts as an immune alarmin. Blood 116: 3465-3474.
3. Zitvogel, L and G Kroemer 2010 The multifaceted granulysin Blood 116: 3376-3380.
4. Zhang H et al 2009 Granulysin induces cathepsin B release from lysosmes of target tumor cells to attck mitochondria through processing of leading to Necroptosis. J. Immunoi 182: 6993-7000.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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