Imgenex Citations of : IMG-321D (Monoclonal%20Antibody%20to%20human%20TLR8%20(Toll-like%20receptor%208)/CD288%20PE%20Conjugate)
1.
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells. Ofer Levy, Eugenie E Suter, Richard L Miller, and Michael R Wessels. Blood, Apr 2006; 10.1182/blood-2005-12-4821. [TLR8-PE, (IMG-321D): Flow cytometry, Fig, 5]
2.
Intracellular Signaling Mechanisms Regulating Toll-Like Receptor-Mediated Activation of Eosinophils. Wong CK, PFY Cheung, WK IP and CWK Lam. Am. J. Respir. Cell Mol. Biol.doi:10.1165/rcmb.2006-0457OC (2007), in press. Imgenex antibodies cited (human blood eosinophils and neutrophils from buffy coat): For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
3.
Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Lee J, CCN Wu, KJ Lee T-H Chuang, K Katakura, Y-T Liu, M Chan, R Tawatao, M Chung, C Shen, HB Cottam, MMC Lai, E Raz and DA Carson. PNAS 103:1828-1833 (2006). Imgenex antibodies cited in this study: Flow (Intracellular) and Flow (Cell Surface) in human Huh-7 hepatoma cells, Fig. 1A [TLR7 (IMG-665A], [TLR8 (IMG-321C)], and [TLR9 (IMG-305D)]; IHC (paraffin) on Huh-7 cells, normal fibroblasts, HCV-infected liver, and liver carcinoma, Fig 1C-E (TLR7, IMG-665A). The specificity of the TLR7 antibody (IMG-665A) was validated using TLR7 293 transfected cells in Flow (Intracellular) (Supplemental Data, Fig. 5).
4.
CD molecules 2006 - Human cell differentiation molecules. Zola H, B Swart, A Banham, S Barry, A Beare, A Bensussan, L Boumsell, CD Buckley, H-J Buhring, G Clark, P Engel, D Fox, B0Q Jin, PJ Macardle, F Malavasi, D Mason, H Stockinger, and X Yang. J Immunological Methods 319:1-5 (2007). Imgenex antibodies cited: TLR8 [IMG-321(A-D)] and TLR10 (IMG-386A). Antibody clones 44C143 (TLR8, IMG-321) and 158C114 (TLR10, IMG-386A) confirmed by the HCDM (Human Cell Differentiation Molecules, previously known as HLDA) 2006 Workshop as TLR8/CD288 and TLR10/CD290, respectively. Also see:http://www.hldaworkshops.org/MoleculeInformation/tabid/54/Default.aspx
5.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid-derived dendritic cells is mediated by IL-12 production. Xu S., U. Koldovsky, M. Xu, D. Wang, E. Fitzpatrick, G. Son, G. Koski, and B. J. Czerniecki., Surgery, 140:170-178 (2006). Imgenex antibodies cited: 1. TLR2 (IMG-416) 2. TLR3 (IMG-315) 3. TLR4 (IMG-417) 4. TLR8 (IMG-321) [Flow (cell surface and intracellular), Fig.1A and 1B (human monocytes)].
6.
Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells. Clark R, Huang S, Murphy G, Mollet I, Hijnen, D, Muthukuru M, Schnabacher C, E Vonetta, Miller D, Kim J, Lambert J, Kupper T. JEM doi: 10.1084/jem.20071190 (2008). Imgenex antibodies cited: 1. TLR8 (IMG-321A): IF/ICC (human squamous cell carcinoma tumor cells), Fig. 3A. 2. TLR7 (IMG-581A): IF/ICC (human squamous cell carcimona tumor cells), Fig. 3A.
7.
STAT3 and STAT5-dependent pathways competitively regulate the pan-differentiation of DC34pos cells into tumor-competent dendritic cells. Cohen P, Koski G, Czerniecki B, Bunting K, Fu X, Wang Z, Zhang W, Carter C, Awad M, Distel C, Nagem H, Paustian C, Johnson T, Tisdale J, Shu S. Immunobiology 112: 1832-1843 (2008). Imgenex antibodies cited [Flow (intracellular), mouse bone marrow cells, Supplementary Fig. S4]: 1. TLR3 FITC (IMG-315C) 2. TLR4 FITC (IMG-5031C) 3. TLR7 (IMG-665A) 4. TLR8 FITC (IMG-321C) 5. TLR9 FITC (IMG-305C)
8.
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures. Sacre S, A Lo, B Gregory, R Simmonds, L Williams, M Feldmann, F Brennan, B Foxwell. J Immunol 181: 8002-8009 (2008). Imgenex antibodies cited [Flow (intracellular), human synovial membrane cells, Fig. 4B]: 1. TLR3-FITC (IMG-315C) 2. TLR8-FITC (IMG-321C) 3. TLR9-FITC (IMG-305C).
9.
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: A novel pathway to autoimmune-associated heart block. Clancy R, D Alvarez, E Komissarova, F Barrat, J Swartz, J Buyon. J Immunol, 184: 2148-2155 (2010). IMGENEX antibodies cited: 1. TLR-7 (IMG-665A): Primary human macrophages derived from PBMCs, Flow (Intracellular): Fig 1A 2. TLR-8 FITC (IMG-321C): Primary human macrophages derived from PBMCs Flow (Intracellular): Fig 1A 3. TLR-7 (IMG-581A): Fetal cardiac fibroblasts, IHC (paraffin) fixed: Fig 7A
10.
TLR Activation pathways in HIV-1 exposed seronegative individuals. Biasin M, L Piacentini, S Caputo, V Naddeo, P Pierotti, M Borelli, D Trabattoni, F Mazzotta, G Shearer, M Clerici. J Immunol 184: 2710-2717 (2010). IMGENEX product cited: TLR8 FITC (IMG-321C): Flow cell surface (CD4+ and CD14+ isolated from PBMC); Fig 3, Fig 4, Table 1.
11.
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. Panda A, F Qian, S Mohanty, D Duin, F Newman, L Zhang, S Chen, V Towle, R Belshe, E Fikrig, H Allore, R Montgomery, A Shaw. J. Immunol 184: 2518-2527 (2010). IMGENEX products cited: 1. TLR9 FITC (IMG-305C) Flow (intracellular) Fig 2, Fig 6, human myeloid dendritic and human myeloid dendritic cells. 2. TLR8 Biotin (IMG-321B) Flow (intracellular) Fig 2, Fig 6, human myeloid dendritic and human myeloid dendritic cells.
12.
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis. Schwab N, A Zozulya, B Kieseier, K Toyka, H Wiendl. J Immunol 184: 5368-5374 (2010). IMGENEX products cited:
TLR7 (IMG-665A): flow (intracellular), human plasmacytoid dendritic cells, data not shown.TLR8 (IMG-321A): flow (intracellular), human plasmacytoid dendritic cells, data not shown.
13.
Toll like receptor triggering in cord blood mesenchymal stem cells. Berk L, Bastiaan J, K Vermeulen, M Netea, T Latuhihin, S Bergevoet, R Raymakers,G Kogler, C Fidgor, G Adema, R Torensma. J Cell Mol Med 13: 3415-3426 (2010). The following antibodies were used in flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
14.
Toll like receptor expression and function in subsets of human T lymphocytes. Pietschmann K, S Beetz, S Welte, I Martens, J Gruen, H Oberg, D Wesch, D Kabelitz. Scandinavian J Immunol 70: 245-255 (2009). 1. TLR4/CD284 (IMG-417A): Flow (cell surface), Human T cell subsets (V sigma 1 and V sigma 2) isolated from PBMCs, Fig 3. 2. TLR6/CD286 (IMG-304A) & TLR8/CD288 PE (IMG-321D): Flow (intracellular), Human T cell subsets (V sigma 1 and V sigma 2) isolated from PBMCs, Fig 3.
15.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. Murciano C, E Vilamon, A Yanez, J Murciano, A Mir, D Gozalbo, M Gil. Immunol & Med Microbiol 51: 327-335 (2007). The following anitbodies were used in flow (cell surface) cytometry: TLR2/CD282 FITC (IMG-416C), TLR4 FITC (IMG-417C), TLR6/CD286 FITC (IMG-304C). Human whole blood from both young and elderly volunteers, Fig 1.
Note: The fluorescence mean intensity was measured in lymphocytes, monocytes, granulocytes (gated by their respective properties in forward and side scatter), Fig 1.
