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QuikChIP Chromatin Immunoprecipitation Kits

 


The Basics...
The principle of the ChIP assay is simple: selective enrichment of a chromatin fraction containing a specific

 
 

target. However, the process can be technically challenging. It is with these challenges in mind that IMGENEX developed the QuikChIP Kit for chromatin immunoprecipitation. QuikChIP™ includes optimized ready-to-use buffers and inhibitory reagents, as well as a comprehensive technical manual.

Quick & simple
Optimized ready-to-use buffers
   & protocol

Useful for Histone and non-histone proteins
• Reagents for 25 ChIP assays and 
  sheared Chromatin preparations

 
 

QuikChIP™ Flow Chart

QuikChIP Certified Antibodies


IMGENEX offers a growing number of antibo-dies that have been validated for use in ChIP assays and we are continually testing and validating new products for use in ChIP. Below is a list of our currently available QuikChIP™ Certified Antibodies. Be sure to check our website often as we are releasing new antibodies every week!

 
 Specificity Host React Application
 ATF6 Mouse Multi ChIP, IF/ICC, IHC-P, IP, WB
 Dnmt1 Mouse M, H WB, IP, ChIP, IHC-P
 Dnmt3a Mouse M, H WB, IHC, ChIP, IF/ICC
 Dnmt3b Mouse M, H WB, IP, ChIP, ICC
 HDAC-3 Rabbit H WB, IP, ChIP
 HDAC-4 Rabbit M, H WB, IP, ChIP
 HDAC-5 Rabbit M, H WB, IP, ChIP
 HDAC-6 Rabbit M, H WB, IP, ChIP
 MBD2 Rabbit M, H WB, FA (Neut), ChIP
 p53 Rabbit M, H, R WB, IP, ChIP, Gel Shift
 p73 Mouse M, H ChIP, IF/ICC, IP, WB
 p73
 (delta-Np73)
Rabbit M, H WB, IHC-P, ChIP
 TRF2 Rabbit M, H WB, IP, ChIP

 

Sheared Chromatin


IMGENEX QuikChIP™ Sheared Chromatin is ready-to-use, sheared, cross-linked chromatin prepared from mammalian cell extracts. This novel product enables researchers to carry out ChIP assays in the absence of cell culture facilities or sonication equipment. It is also useful as a sheared, cross-linked chromatin positive  control, alongside your own cell extracts.

 Conditions for cell culture, DNA-protein cross-linking, and the sonication shearing process have been optimized for each human and mouse cell type. 

  Sheared Chromatin Extract Cat. No.
  293  Sheared Chromatin 30304
  3T3  Sheared Chromatin 30306
  A375  Sheared Chromatin 30309
  A549  Sheared Chromatin 30310
  CP70  Sheared Chromatin 30311
  Daudi  Sheared Chromatin 30308
  HeLa  Sheared Chromatin 30301
  Jurkat Sheared Chromatin 30302
  MCF7  Sheared Chromatin 30307
  RAW  Sheared Chromatin 30303
  THP1  Sheared Chromatin 30305
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