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Monoclonal Antibody to CD196 (CCR6), Clone IMG18B9E6

Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
CD196 (CCR6)Purified
0.025 mg
0.1 mg
Catalog No: IMG-6918A
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Contents: CD196 (CCR6) antibody at 0.5 mg/ml in PBS, 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rat IgG2a, Kappa
Clone: IMG18B9E6
Purification: Protein G Chromatography was used to purify the CD196 (CCR6) antibody.
Species: Mouse
Predicted React: Other species not tested
Host: Rat
Application: IHC (p): use antibody at 5 ug/ml
Western blot analysis: 2-5 ug/ml
Storage: Store CD196 antibody at 4°C, stable for 6 months. For long-term storage, aliquot antibody and store at -20°C.

CD196 (CCR6) is a chemokine receptor expressed on Th17 cells and regulatory Tcells that is induced by T-cell priming with certain cytokines. This protein plays a pivotal role in directing inflammatory and regulatory cells to inflamed tissues or the gut, where the ligand of CD196, CCL20 is expressed. Although most chemokine-chemokine receptor interactions are characterized by a high promiscuity, CD196 binds only CCL20 and β-defensins. This chemokine receptor is widely expressed on human blood and tissue leukocytes, including subsets of dendritic cells, CD45RO+ effector/memory Tcells, CD25 high regulatory Tcells (Tregs), naive and memory B cells, NKT cells, and NK cells. On memory Tcells, CD196 is expressed on a population of autoreactive interleukin-10 (IL-10)–producing cells and subsets of skin- and mucosa-homing cells. More recently, CD196 was identified as a new risk factor for rheumatoid arthritis; moreover, a nucleotide polymorphism within the Cd196 locus was shown to be associated with susceptibility to rheumatoid arthritis, Graves disease, and Crohn disease (Steinfelder S et al, .2011).

This protein also acts as a marker for Th17 lineage polarization and HIV permissiveness in memory CD4+ Tcells. CD196+ Tcells have the potential to migrate into the GALT via the gut-homing integrin α4β7, a newly identified HIV-gp120 binding receptor. Nevertheless, given the ability of integrin β7 to regulate cell migration into the GALT and bind HIV-gp120, CD196+ Tcells coexpressing integrin β7 and CCR5 might have an extraordinary ability to disseminate HIV from the portal sites of entry (Monteiro P et al ,. 2011). Anti-CD196 antibody could almost completely suppress the cell migration induced by hBD-2 (Human beta-defensin-2), suggesting that hBD-2 mainly utilizes CD196 (CCR6) as a functional receptor (Niyonsaba F et al,.2004).

A recombinant protein consisting of the external domains of mouse CD196 (CCR6) was used as the immunogen for the antibody.

Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Genebank Info (Protein): NP_001177262 
Gene ID: 12458
SwissProt: O54689
CD196 CCR6 Antibody WB

Western blot analysis of A) mouse EL4, B) conA treated EL4 lysate and C) partial recombinant protein using CD196 (CCR6) antibody at 2 ug/ml.

CD196 CCR6 Antibody IHC

Formalin-fixed, paraffin-embedded normal mouse testis tissue stained with CD196 (CCR6) antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining. IMGENEXs mouse organ tissue array (IMG-335) was used for this test.


1. Niyonsaba F, Ogawa H, Nagaoka I. Immunology. 2004 Mar;111(3):273-81. PMID: 15009427
2. Monteiro P, Gosselin A, Wacleche VS, El-Far M, Said EA, Kared H, Grandvaux N, Boulassel MR, Routy JP, Ancuta P. J Immunol. 2011 Apr 15;186(8):4618-30. doi: 10.4049/jimmunol.1004151. Epub 2011 Mar 11. PMID: 21398606
3. Steinfelder S, Floess S, Engelbert D, Haeringer B, Baron U, Rivino L, Steckel B, Gruetzkau A, Olek S, Geginat J, Huehn J, Hamann A. Blood. 2011 Mar 10;117(10):2839-46. doi: 10.1182/blood-2010-06-293027. Epub 2011 Jan 12. PMID: 21228329

Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.






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