PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : >>
      
 

Monoclonal Antibody to CD196 (CCR6), Clone IMG18B9E6

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6918A-25UG
IMG-6918A-0.1MG
CD196 (CCR6)Purified
0.025 mg
0.1 mg
--
 --
 
Catalog No: IMG-6918A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: CD196 (CCR6) antibody at 0.5 mg/ml in PBS, 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rat IgG2a, Kappa
Clone: IMG18B9E6
Purification: Protein G Chromatography was used to purify the CD196 (CCR6) antibody.
Species: Mouse
Predicted React: Other species not tested
Host: Rat
Application: IHC (p): use antibody at 5 ug/ml
Western blot analysis: 2-5 ug/ml
Storage: Store CD196 antibody at 4°C, stable for 6 months. For long-term storage, aliquot antibody and store at -20°C.
 

Background
CD196 (CCR6) is a chemokine receptor expressed on Th17 cells and regulatory Tcells that is induced by T-cell priming with certain cytokines. This protein plays a pivotal role in directing inflammatory and regulatory cells to inflamed tissues or the gut, where the ligand of CD196, CCL20 is expressed. Although most chemokine-chemokine receptor interactions are characterized by a high promiscuity, CD196 binds only CCL20 and β-defensins. This chemokine receptor is widely expressed on human blood and tissue leukocytes, including subsets of dendritic cells, CD45RO+ effector/memory Tcells, CD25 high regulatory Tcells (Tregs), naive and memory B cells, NKT cells, and NK cells. On memory Tcells, CD196 is expressed on a population of autoreactive interleukin-10 (IL-10)–producing cells and subsets of skin- and mucosa-homing cells. More recently, CD196 was identified as a new risk factor for rheumatoid arthritis; moreover, a nucleotide polymorphism within the Cd196 locus was shown to be associated with susceptibility to rheumatoid arthritis, Graves disease, and Crohn disease (Steinfelder S et al, .2011).

This protein also acts as a marker for Th17 lineage polarization and HIV permissiveness in memory CD4+ Tcells. CD196+ Tcells have the potential to migrate into the GALT via the gut-homing integrin α4β7, a newly identified HIV-gp120 binding receptor. Nevertheless, given the ability of integrin β7 to regulate cell migration into the GALT and bind HIV-gp120, CD196+ Tcells coexpressing integrin β7 and CCR5 might have an extraordinary ability to disseminate HIV from the portal sites of entry (Monteiro P et al ,. 2011). Anti-CD196 antibody could almost completely suppress the cell migration induced by hBD-2 (Human beta-defensin-2), suggesting that hBD-2 mainly utilizes CD196 (CCR6) as a functional receptor (Niyonsaba F et al,.2004).

Antigen
A recombinant protein consisting of the external domains of mouse CD196 (CCR6) was used as the immunogen for the antibody.

Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Genebank Info (Protein): NP_001177262 
Gene ID: 12458
SwissProt: O54689
 
CD196 CCR6 Antibody WB

Western blot analysis of A) mouse EL4, B) conA treated EL4 lysate and C) partial recombinant protein using CD196 (CCR6) antibody at 2 ug/ml.

CD196 CCR6 Antibody IHC

Formalin-fixed, paraffin-embedded normal mouse testis tissue stained with CD196 (CCR6) antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining. IMGENEXs mouse organ tissue array (IMG-335) was used for this test.

-

Reference
1. Niyonsaba F, Ogawa H, Nagaoka I. Immunology. 2004 Mar;111(3):273-81. PMID: 15009427
2. Monteiro P, Gosselin A, Wacleche VS, El-Far M, Said EA, Kared H, Grandvaux N, Boulassel MR, Routy JP, Ancuta P. J Immunol. 2011 Apr 15;186(8):4618-30. doi: 10.4049/jimmunol.1004151. Epub 2011 Mar 11. PMID: 21398606
3. Steinfelder S, Floess S, Engelbert D, Haeringer B, Baron U, Rivino L, Steckel B, Gruetzkau A, Olek S, Geginat J, Huehn J, Hamann A. Blood. 2011 Mar 10;117(10):2839-46. doi: 10.1182/blood-2010-06-293027. Epub 2011 Jan 12. PMID: 21228329


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways